Signalling

Part:BBa_K5399005:Design

Designed by: josiazhang josiazhang   Group: iGEM24_NAIS   (2024-09-29)


NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6
    Illegal NgoMIV site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NLS functions to facilitate the transport of proteins into the nucleus by interacting with nuclear transport receptors. This signal is crucial for the nuclear localization of Cas9 proteins, enabling it to perform essential roles in SBEI and SBEII gene knockout.


Source

We obtained the NLS sequence from the psgR-Cas9-At vector.

References